Cellular Internalization and RNA Regulation of RNA Virus

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Cellular Internalization and RNA Regulation of RNA Virus


Shihori Tanabe*


Senior Researcher, Division of Risk Assessment, Center for Biological Safety and Research, National Institute of Health Sciences, Japan

*Corresponding author: Shihori Tanabe, Senior Researcher, Division of Risk Assessment, Center for Biological Safety and Research, National Institute of Health Sciences, Japan

Citation: Tanabe S. (2020) Cellular Internalization and RNA Regulation of RNA Virus. Adv Clin Med Res. 1(1):1-3.

Received: April 4, 2020, | Published: May 11, 2020

Copyright© 2020 genesis pub by Tanabe S. CC BY-NC-ND 4.0 DEED. This is an open-access article distributedunder the terms of the Creative Commons Attribution-NonCommercial-No Derivatives 4.0 International License.,This allows others distribute, remix, tweak, and build upon the work, even commercially, as long as they credit the authors for the original creation.

DOI: https://doi.org/10.52793/ACMR.2020.1(1)-02


Biological responses are regulated by various molecular networks. It is significantly important to reveal the mechanisms of diseases and cellular responses for the prevention and drug development. The infection mechanism of RNA virus consists of viral internalization, replication including RNA transcription, and expansion. RNA regulation of the RNA virus is critical for the replication of RNA virus. The therapy for the infectious diseases may target the viral internalization, replication and expansion. In this Editorial, the internalization and replication mechanism of RNA virus, especially novel corona virus SARS-CoV-2 which causes infectious diseases, is focused on and described.


Novel coronavirus; RNA virus; RNA viral infection

Mechanism of Viral Infection

Coronavirus, which is a nanoparticle, is sphere-shaped and its diameter is 80-120 nm in average, where it sometimes ranges from 50 nm to 200 nm [1]. Spike protein (S protein), so-called peplomer, on the surface of the particle binds to the receptor on the host cellular membrane, then internalized inside the cells. Viral RNA (plus strand) in the viral particles is replicated and translated into the viral structure protein in the host cells, which is followed by replication of new viral particles [2]. Coronavirus is recognized by the binding of S protein on the viral surface and angiotensin I converting enzyme 2 (ACE2) receptor on the cellular membrane, then internalized into the cell via processing of S protein by transmembrane serine protease 2 (TMPRSS2) protease [3].

Therapeutic Targets for RNA Virus

The inhibition of this internalization of the viral particle would theoretically prevent the viral infection and replication, which suggests the drug development targeting the internalization of the viral particle. The receptor recognition mechanism of this S protein on the surface of the nano-viral particle needs to be elucidated, which may lead to the identification of the target molecules of RNA viral infection to treat or prevent diseases. ACE2 has been identified as receptor for corona virus (SARS-CoV) [4]. ACE2, the first human homologue of ACE, functions as a carboxypeptidase on the cellular membrane, and hydrolyses angiotensin II, which plays an important role in renin-angiotensin system, to angiotensin (1-7) [5]. ACE 2 is highly expressed in gastrointestinal system such as small intestine and duodenum, as well as oral and nasal mucosa, lung, kidney and brain [6-8]. The interaction between ACE2 and S protein on the surface of viral particle may be a potential target to prevent coronaviral infection. Regarding the development of coronaviral vaccine, the sequences of S protein may be a crucial key for the recognition of the antigen by antibody. It has been revealed that SARS-CoV-2 has similar protein sequences to other RNA viruses which bind to ACE2 in the binding motif of S proteins. The coronavirus has viral RNA genome inside, of which RNA will be replicated by RNA polymerase [9,10]. The RNA polymerase inhibition is another target to treat the disease, while the molecular network of the RNA regulation mechanism needs to be examined for the safe application of RNA polymerase inhibitors.


The RNA viral infection consists of internalization, replication and expansion, which may be therapeutic targets for the treatment of the infectious disease. It would be the great advancement to understand the RNA virus strategy and biological molecular network responses.



  1. Masters PS. (2006) The molecular biology of coronaviruses. Adv Virus Res. 66:193-292.
  2. Weiss SR, Navas-Martin S. (2005) Coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus. Microbiol Mol Biol Rev. 69(4):635-64.
  3. Hoffmann M, Kleine-Weber H, Schroeder S, Krüger N, Herrler T, et al. (2020).SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. 181(2):271-280.e278.
  4. Hamming I, Timens W, Bulthuis ML, Lely AT, Navis G. (2004) Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in understanding SARS pathogenesis. J Pathol. 203(2):631-37.
  5. Warner FJ, Smith AI, Hooper NM, Turner AJ. (2004) Angiotensin-converting enzyme-2: a molecular and cellular perspective. Cell Mol Life Sci. 61(21):2704-13.
  6. Fagerberg L, Hallström BM, Oksvold P, Kampf C. Djureinovic D, et al. (2014) Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics. Mol Cell Proteomics. 13(2):397-406.
  7. Farmer D, Gilbert M, Borman R, Clark KL. (2002) Quantitative mRNA expression profiling of ACE 2, a novel homologue of angiotensin converting enzyme. FEBS Lett. 532(1-2):107-110.
  8. Xu H, Zhong L, Deng J, Peng J, Dan H,  et al. (2020) High expression of ACE2 receptor of 2019-nCoV on the epithelial cells of oral mucosa. Int J Oral Science. 12(1):8.
  9. Schoeman D, Fielding BC. (2019) Corona virus envelope protein: current knowledge. Virol J. 16(1):69.
  10. Warner FJ, Smith AI, Hooper NM, Turner AJ. (2004) Angiotensin-converting enzyme-2: a molecular and cellular perspective. Cell Mol Life Sci. 61(21):2704-13.